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. Author manuscript; available in PMC: 2010 Jan 11.
Published in final edited form as: J Biol Chem. 2007 Feb 28;282(17):12725–12733. doi: 10.1074/jbc.M611909200

FIGURE 1. Proteasome inhibitors induce ER stress in Neuro2A cells.

FIGURE 1

A, N2A-mPrP cells were treated with 15 μM MG132 (MG) or 6 μM epoxomycin (Epox) for 16 h, and the levels of PrP, GADD153/CHOP, Grp58, Grp78/Bip, and pro-caspase-12 were determined by Western blot. B, Neuro2A cells stably transfected with a dominant negative form of caspase-12 (caspase-12DN) or empty vector (mock) were treated with 6 μM epoxomycin (Epox), 15 μM lactacystin (Lacta), or 15 μM MG132, and after 24 h of incubation cell viability was determined by the MTS assay. Data show the mean ± S.D. of two different experiments performed in triplicate. NT, non-treated cells.