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. Author manuscript; available in PMC: 2010 Jan 11.
Published in final edited form as: Nat Neurosci. 2007 Apr 8;10(5):578–587. doi: 10.1038/nn1893

Figure 1. FMRP interacts directly with the 3′UTR of PSD–95 mRNA.

Figure 1

(a) Brain lysates from wildtype (WT) and FMR1 knockout mice (KO) were immunoprecipitated with FMRP antibodies. RT–PCR was performed using oligos for the PSD–95, MAP1B and GluR1 mRNAs. Input (1/5) is reported in lanes 2. Lanes not relevant to this experiment were removed between the marker and lanes 1 and 2. (b) CLIP assay. Hippocampal cell extracts were immunoprecipitated with FMRP antibodies. RT–PCR was performed using oligos for the PSD–95, MAP1B and GlyRα mRNAs. Input (1/5) is reported in lanes 2, 5, 8. (c) PSD–95 3′UTR fragments utilized in EMSA experiments. Potential functional motifs are indicated. (d) 32P radiolabelled fragments (1–5) of the PSD-95 3′UTR were incubated in the presence of FMRP (+, lanes 2, 4, 6, 8, 10). Control reactions were performed in buffer alone (−, lanes 1, 3, 5, 7, 9). RNA:protein complexes were resolved on native polyacrylamide gel. Unbound RNA fragments (]), and RNA:protein complexes (*) are indicated.