FIGURE 1.

THC and CBD inhibit the LPS-induced release of IL-1β and IL-6 from BV-2 cells. Cells were preincubated for 2 h with the indicated concentrations of THC or CBD and then activated for 4 h with 100 ng/ml LPS. Cell-free media were then collected, and the release of IL-1β (A) and IL-6 (B) was measured using ELISA. The percentage compared with LPS applied alone (marked as 100%) is expressed as the mean ± S.E. of three independent experiments. One-way ANOVA was used as follows: IL-1β, F(7,16) = 10.21, p < 0.001 and IL-6 F(7,16) = 81.8, p < 0.0001. Bonferroni post hoc test: *, p < 0.05; **, p < 0.01; ***, p < 0.001 show significant differences from LPS-treated cells.