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. 2009 Nov 20;285(3):1627–1633. doi: 10.1074/jbc.M109.080556

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FIGURE 3. — Fluorescent labeling of apo-FDH by recombinant PPT. A, SDS-PAGE of recombinant apo-FDH incubated with recombinant PPT and fluorescent reporter for different periods of time. Upper panel, fluorescence of the gel after UV irradiation. Lower panel, Coomassie stain of the same gel. B, quantification of the fluorescence from A. C, fluorescence labeling of wild-type and S354A mutant apo-FDH by recombinant PPT. Upper panel, fluorescence under UV irradiation of SDS-PAGE. Lower panel, Coomassie stain of the same gel.