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. 2009 Nov 13;285(3):1653–1660. doi: 10.1074/jbc.M109.051185

FIGURE 1.

FIGURE 1.

Transition to a steady-state GLUT4 distribution in L6 myotubes. A, L6 myotubes were stimulated with insulin (●), AICAR (■), A-769662 (▴), and insulin + AICAR (○) for the indicated times, and then cells were fixed, and the surface GLUT4 level was determined in fixed cells using an HA primary antibody and an Alexa 488 secondary antibody. Each data point represents the mean ± S.E. from three experiments. In some cases, the error bars are smaller than the symbol. B, times for the half-maximal increase above basal in cell surface HA-GLUT4 were determined for insulin, AICAR, A-769662, and the combination of insulin and AICAR. C, cell surface levels of GLUT4 reached at 30 min of incubation with the agonists were determined as a percentage of the total cellular GLUT4 available in permeabilized cells. D, plateau levels of antibody labeled-GLUT4 reached at 180 min of incubation with the anti-HA antibody were determined as a percentage of the total cellular GLUT4 available in permeabilized cells. Results are the mean ± S.E. from three experiments.