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Kinetic parameters for GLUT4 recycling at steady state. The uptake time courses for the increase in antibody bound to GLUT4 HA were analyzed to give the following: A, plasma membrane level of HA-GLUT4, P; B, total available GLUT4, T; C, kex rate constant; and D, kin rate constant. WM, wortmannin. The analyzed data are from 3 to 7 experiments. Standard errors calculated from the confidence intervals (see “Experimental Procedures”) for the fits are shown.
