FIGURE 4.

Effect of anti-HS antibodies on the interaction of CyPB with cell surface HS. Jurkat T cells (A) and THP-1 cells (B) were preincubated in the presence of anti-HS antibodies or isotype control and allowed to adhere to immobilized CyPB (1 μg/well). Cell binding was related to the number of initially added cells (0.8 × 10⁶ per well) remaining fixed to the adhesive substrate. Heparinase-treated cells were used as a control to estimate the participation of HS in the interaction. Maximal binding obtained in the absence of antibody was estimated at 0.48 ± 0.08 × 10⁶ and 0.33 ± 0.05 × 10⁶ cells per well for Jurkat and THP-1, respectively. Results are normalized to these control values, which were set at 100%. Each bar of the histograms represents the mean ± S.D. of triplicates from three separate experiments. *, significant difference compared with isotype control (p < 0.05).