FIGURE 7.
BAEBL secreted from P. falciparum also binds to heparin. Culture supernatants of the P. falciparum HB3 clone were incubated with heparin-agarose, Ni2+-NTA-agarose or glutathione-Sepharose for 3.5 h at 4 °C. The culture supernatants (Sup) of P. falciparum-infected and uninfected erythrocytes and the eluates (Elu) from the washed beads were separated on 8% SDS-PAGE and transferred to a polyvinylidene difluoride membrane. The specific band (arrowhead) for BAEBL was detected using a rabbit anti-BAEBL polyclonal antibody. The molecular masses (kDa) are indicated on the left.