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. 2009 Nov 3;285(3):1726–1732. doi: 10.1074/jbc.M109.046847

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FIGURE 2. — IGFBP-3 was detected in testicular mitochondrial fraction by ELISA only after anti-BAX IP. A, rats were treated with vehicle (Control), GnRH-A, IGFBP-3, or GnRH-A plus IGFBP-3 peptide as described under “Experimental Procedures.” IGFBP-3 levels measured by ELISA were very low and not different in testicular mitochondrial fractions without anti-BAX co-immunoprecipitation after the various treatments. B, quantitative measurements of IGFBP-3 in testicular mitochondrial fractions after anti-BAX co-immunoprecipitation showed no detectable IGFBP-3 in the basal control state and significant increases in IGFBP-3 after treatment with GnRH-A, IGFBP-3, or combination of both peptides compared with control group. Values are mean ± S.D. (error bars) (n = 4 testis homogenates). *, p < 0.05.