TABLE 2.

Measured kinetic and thermodynamic parameters of the interaction of Ca²⁺-saturated CaM with melittin or with SPC

Experiment	Parametera	Value	Dimension	Fig.
CaM-ME binding	k+1,M	1004 ± 366	μm−1 s−1	1B
	k−2,M + k+2,M	49 ± 13	s−1	1B
	Kd1,M	0.3 ± 0.15	μm	1C
	Kd1–2,M	0.067 ± 0.044	μm	1C
	Kdeq,M	0.054 ± 0.016	μm	1D
CaM-SPC binding	k+1,S	>40	μm−1 s−1
SPC micelle formation	CMC	33 ± 2	μm	Ref. 14
SPC chase	Kd1–2,SC	32 ± 0.7	μm	2B
	Kd1,SC	51 ± 3	μm	2B
	k+1,SC	2.4 ± 0.2	μm−1 s−1	2C
	k1,sat,SC	348 ± 30	s−1	2C
	k2,obs,SC	14 ± 6	s−1	2C
	k3,obs,SC	0.9 ± 0.3	s−1	2C
ME chase	Kd1–2,MC	1.0 ± 0.3	μm	3B
	k1,sat,MC	59 ± 13	s−1	3C
	K1(app),MC	5.4 ± 3.4	μm	3C
	K2,obs,MC	4–8	s−1	3C

^a Explanation of the indexes: M, parameter obtained for CaM-melittin binding; S, parameter obtained for CaM-SPC binding; C, parameter obtained in a chasing experiment; ± signs and numbers indicate specific reaction steps as shown in Scheme 1 and Fig. 8.