FIGURE 3.

β3(D723R) increases αIIbβ3 binding to PAC-1 IgM and Fab. Binding of PAC-1 IgM (10 μg/ml) was followed by washes and analysis of live unfixed cells. Binding of PAC-1 Fab (20 μg/ml) was done with a fixation step prior to washing. Values are as in Fig. 1 but are the mean ± S.E. (error bars) for 4 and 5 experiments for the IgM and Fab samples, respectively. The difference between PAC-1 Fab binding to the cell lines expressing wild type (WT) β3 and β3 (D723R) (D>R) is significant (p = 0.011).