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PAC-1 IgM binding to CHO cells is clustered. PAC-1 IgM bound to CHO cells expressing αIIbβ3 and GFP-talin head F2-F3 domain was fixed, labeled with fluorescent secondary antibodies, and visualized by epifluorescence microscopy. A, maximum intensity projection image of a cell showing clustered PAC-1 IgM on the cell surface. PAC-1 IgM fixed to a glass slide and detected with secondary antibodies show significantly less clustering (B).
