Skip to main content
. 2009 Nov 2;285(3):1850–1860. doi: 10.1074/jbc.M109.023762

FIGURE 1.

FIGURE 1.

Expression of Survivin in human leukemia cell lines and induction of Survivin by E2A-HLF in human ALL cells. A, top 2 panels, Northern blot analysis of poly(A)+ RNA isolated from human leukemia cell lines. The blot was hybridized with a survivin cDNA probe and then rehybridized with a β-actin probe. Lower three panels, immunoblot analysis using whole-cell lysates. Survivin, E2A-HLF, and α-tubulin proteins were detected with specific antibodies. Lanes 1–4, the UOC-B1, YCUB-2, HAL-O1, and Endo-kun t(17;19)-positive pro-B ALL cell lines; lanes 5–8, the RS4;11, Nalm-6, REH, and 920 pro-B ALL cell lines without t(17;19); lane 9, the Jurkat T-ALL cell line; lane 10, the HL-60 AML cell line; and lane 11, the NB-4 APL cell line. B, Nalm-6 cells with zinc-inducible expression of E2A-HLF (Nalm-6/E2A-HLF) and control Nalm-6/pMT cells were cultured in medium containing 100 μm zinc for the indicated length of time. C and D, UOC-B1 cells with zinc-inducible expression of E2A-HLF(dn) (UOC-B1/E2A-HLF(dn)) and control UOC-B1/pMT cells were cultured in medium containing 100 μm zinc for the indicated length of time (Zn(−) → Zn(+)) and removal of zinc from the growth medium (Zn(+) → Zn(−)). C, upper two panels, Northern blot analysis of poly(A)+ RNA. The blot was hybridized with a survivin cDNA probe and then rehybridized with a β-actin probe. Lower three panels, immunoblot analysis for Survivin, E2A-HLF, or α-tubulin proteins. D, quantification of intensity of each band.