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Effect of calmodulin on CN585-mediated calcineurin inhibition. Calcineurin inhibition by various CN585 concentrations was measured in the presence of 50 nm (square), 100 nm (diamond), and 200 nm (triangle) calmodulin using the RII phosphopeptide substrate. Initial rates of calcineurin activity were plotted against the inhibitor concentration. The data presented are the means ± S.D. of three independent experiments.
