FIGURE 6.

Influence of CN585 on NFAT translocation in HeLa cells. HeLa cells were transfected with a green fluorescent protein-NFAT^2–417 expression plasmid by electroporation. Ten hours after transfection cells were incubated for 45 min with CN585, CN675, or CsA as positive and [MeVal]⁴CsA as negative controls. Then cells were stimulated with PMA/ionomycin for 1 h, paraformaldehyde-fixed, and 4′,6-diamidino-2-phenylindole-stained. The fluorescence images were recorded with a CCD camera upon Nikon TE200 epifluorescence microscope. The experiment was repeated two times with similar results.