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. 2009 Nov 12;285(3):1909–1918. doi: 10.1074/jbc.M109.058487

FIGURE 1.

FIGURE 1.

Msc1 is an integral component of the fission yeast Swr1 complex. A, Msc1-FLAG is functional. The loss rates of a nonessential chromosome (Ch16) in the indicated strains were measured. wt, wild type. B, cell extract prepared from an Msc1-FLAG strain was immunoprecipitated with anti-FLAG resin and subjected to Western blot analysis with anti-FLAG antibody. C, cell extract prepared from an Msc1-FLAG strain was used to perform affinity purification with anti-FLAG resin, resolved by SDS-PAGE, and silver-stained. D, shown are the results from MudPIT mass spectrometry analysis of proteins associated with Msc1-FLAG. The numbers of peptides, as well as the percentages of each protein these peptides cover, are indicated. The corresponding proteins of the S. cerevisiae Swr1 complex were also shown for comparison. E, protein extracts prepared from the indicated strains were immunoprecipitated (IP) with anti-FLAG resin and analyzed by Western blot analysis with anti-FLAG and anti-Myc antibodies. F, an affinity-purified complex containing Msc1-FLAG and Swr1-Myc was subjected gel filtration analysis with a Superose 6 column. Fractions were trichloroacetic acid-precipitated and subjected to Western blot analysis with anti-FLAG and anti-Myc antibodies. The elution volumes of protein standards (in kilodaltons) are also indicated.