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. 2009 Nov 12;285(3):1909–1918. doi: 10.1074/jbc.M109.058487

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FIGURE 4. — Msc1, Swr1, and H2A.Z^Pht1 are required for proper chromosome segregation. A, Msc1 and Swr1 are required for the maintenance of a nonessential minichromosome. wt, wild type. B, shown is a diagram of the fission yeast centromere 1. The insertion site of the cnt1::ura4⁺ reporter gene is indicated. C, serial dilution plating analysis of the indicated strains were performed to measure the expression of ura4⁺. FOA, 5-fluoroorotic acid; N/S, non-selective medium. D, RT-PCR analyses were performed to measure the RNA levels of ura4⁺ and act1⁺. −RT indicates the reverse transcription step was omitted. E, ChIP analysis was performed to measure the levels of Cnp1-FLAG at centromere regions. -Fold enrichment is shown below each lane. F, shown are the results from live cell imaging analysis of the indicated yeast strains expressing Cnp1-GFP.