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H2A.ZPht1 regulates CENP-CCnp3 expression. A, ChIP-chip analysis of H2A.ZPht1 enrichment at the centromere of chromosome 2 (cnt2) and a 10-kb region that includes cnp3+. wt, wild type. B, ChIP analysis of H2A.ZPht1 at centromeres (cnt) and the cnp3 promoter. C, RT-PCR analysis of Cnp3 mRNA levels. D, CENP-CCnp3 is required for silencing at centromeres. RT-PCR analysis were performed to measure the expression of cnt1::ura4+.
