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. 2009 Nov 12;285(3):1909–1918. doi: 10.1074/jbc.M109.058487

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FIGURE 6. — PHDs are required for Msc1 function. A, shown is the domain architecture of Msc1. Zf, zinc finger. B, shown is sequence alignment of the JmjC domains of the JARID1 family proteins. Arrows indicate amino acid residues mutated in Msc1. Asterisks represent residues that are essential for histone demethylase activity (17, 60). sp, S. pombe; hs, Homo sapiens; dm, Drosophila melanogaster. C, cell extracts prepared from the indicated strains were subjected to Western blot analysis with anti-Myc antibody. Ponceau S staining of part of the membrane is shown below as a control for loading. wt, wild type. D, shown is the maintenance of a nonessential minichromosome in Msc1 mutants. E, serial dilution plating analysis of the indicated strains was performed to measure the expression of cnt1::ura4⁺. FOA, 5-fluoroorotic acid; N/S, non-selective medium.