FIGURE 5.

LMP loss in ZOL-induced apoptosis of HF28RA cells is regulated by BclXL and caspase-9. Cells were exposed to 50 μm ZOL for the times indicated, and lysosomal stability was assessed using the acridine orange uptake method. Results are expressed as the percent of cells with low AO staining and are representative of a minimum of three independent determinations. Data are expressed as the mean values ± S.E. (n = 3–6) and analyzed by two way ANOVA with the Bonferroni post-test. ***, p < 0.001 versus corresponding ZOL treatment effects on LMP of HF28RA GFP cells; □□□, p < 0.001 for the comparison of ZOL exposure effects on LMP of HF28RA BclXL versus ZOL exposure effects on HF28RA DN9 cells. CTL, control.