FIGURE 2.

FPP-activated GR is transcriptionally active. A and B, transfection experiments of HEK with the K6-CAT and GRE-CAT reporters are shown. The data are presented as relative CAT activity, a measure of actual CAT activity normalized to total protein. The results show that DEX and FPP treatment lead to repression of the K6 promoter and stimulation of the GRE-CAT reporter. The effect of both DEX and FPP was reversed with RU486 antagonist of GR. C, quantitative analysis of immunoblotting with anti-Ser(P)²¹¹ GR antibody normalized to GAPDH and plotted as fold change in GR phosphorylation. HEK were incubated in the presence or absence of 1 μm DEX, 10 μm FPP, 10 μm RU486, or a combination for 4 h. Both DEX and FPP induce an increase in Ser²¹¹ GR phosphorylation, which was reversed with RU486.