FIGURE 2.

Intracellular localization of SOUL. A, immunofluorescent confocal microscopy analysis of GFP-SOUL in NIH3T3 cells. Cells were transfected with pEGFP-C1 plasmid containing the full-length SOUL open reading frame (2) or with pEGFP-C1 plasmid alone (1). Representative images of three independent experiments are presented. B, Western blot analysis of cytosolic (C), mitochondrial (M), and nuclear (N) fractions of HeLa cells treated (H₂O₂) or not (Control) with 100 μm H₂O₂ for 24 h. Western blotting was performed as described previously utilizing respective primary antibodies recognizing SOUL, the cytosolic marker glyceraldehyde-3-phosphate dehydrogenase (GA3PD), the mitochondrial marker pyruvate decarboxylase-1α (PDC-1α), and the nuclear marker histone H1 (Histon H1). Photomicrographs demonstrate representative blots of three independent experiments.