FIGURE 8.

Complement regulatory activity of FH purified from the ejaculated sperm membrane. A, SDS-PAGE of FH purified from the ejaculated sperm membrane (lane 1). FH was solubilized with 2% sodium cholate/PBS treatment and purified using an anti-FH immunoaffinity column. Purified FH from PSP was loaded as a control (lane 2). B, comparison of complement regulatory activities of FHs from ejaculated sperm membrane (open circles) and PSP (filled circles). The assay was carried out on an LPS-coated plate as described under “Experimental Procedures.” Porcine albumin (open squares) was used as a negative control. Similar results were obtained from at least three separate experiments. Each value is the mean ± S.E. from triplicate experiments.