TABLE 1.

Purification of FH from porcine seminal plasma

Step	Volume	Total absorbance (280 nm)a	Factor Hb	Yield	Purification
	ml		mg	%	-fold
Dialysis	550	10,302	30	100	1
Q-Sepharose	175	142	28	93	67
Matrex Red A	280	42	16	52	128
Superdex 200	48	8.4	6.7	22	274

^a Protein concentration was measured by absorbance at 280 nm in a 1-cm light path, and 1 mg of protein was defined as the concentration required to yield an absorbance of 1.4 (E_{280 nm}^0.1% = 1.4).

^b The concentration of factor H was determined by the method of Mancini (28).