FIGURE 5.

A fluorescent binding analysis on living cells reveals differential FGF binding preferences of human FGFRL1. HEK293 cells expressing FGFRL1 were incubated with selected fluorescently labeled FGFs (DyLight 547, red channel). The subcellular localization of FGFRL1 (Cy-2, green channel) is shown in the top panel. The right panels show the cell nuclei after staining with 4′,6′-diamino-2-phenylindole. Note that FGF2 and FGF3 bind to the surface of the FGFRL1 expressing cells, whereas FGF1 and FGF12 do not bind. Bar, 20 μm.