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. 1998 Dec 22;95(26):15339–15344. doi: 10.1073/pnas.95.26.15339

Figure 2.

Figure 2

Stabilization of β-catenin by N-cadherin derivatives. (A) Western blot of proteins from CHO cells (control), and cells stably expressing N-cadherin (N-cad), the IL-2R/N-cadherin chimera (IL-2R/N-cad), or the N-cadherin cytoplasmic tail [N-cad (tail)] with anti-N-cadherin (lanes 1 and 2), the IL-2R (lane 3), and Flag (lane 4). An identical blot was probed with anti-β-catenin antibody. (B) Co-IP with anti-cadherin (cad) from CHO cell extracts (lane 1) and from cells transfected with N-cadherin (lane 2), with anti-IL-2R from cells transfected with IL-2R/N-cadherin (IL-2R, lane 3), or anti-flag from cells transfected with the N-cadherin tail (flag, lane 4). Shown is an immunoblot (IB) of the IPs with anti-cadherin or anti-β-catenin antibodies. The bands around 25 kDa and 50 kDa in all lanes are light and heavy Ig chains from the IP. (C) Triton X-100 solubility of proteins from CHO cells expressing N-cadherin and the N-cadherin cytoplasmic tail. Triton X-100-soluble (sol) and -insoluble (ins) fractions of cell extracts were immunoblotted with anti-cadherin or anti-flag antibodies.