Table 3.

ACh-induced changes in cell membrane properties

			Changes in Membrane Properties
	Control		Mean
	Total	EGFP+	Total	EGFP+	↑Em Total	↓Em Total
n	19	15	19	15	10	6
Em, mV	−68.7 ± 7	−69.3 ± 7	3.7 ± 9†	3.1 ± 9†	10.4 ± 7*	−5.7 ± 5†
Ith, pA	73.7 ± 31	66.7 ± 24	2.6 ± 48	10.0 ± 48	−10.0 ± 39	25 ± 69
Rin, MΩ	426.7 ± 241	446.7 ± 242	−56.4 ± 78*	−69.6 ± 78†	−60.9 ± 92†	−37.2 ± 68
Vth, mV	−40.7 ± 10	−41.8 ± 8	−3.9 ± 4*	−3.3 ± 3*	−3.4 ± 4†	−5.6 ± 3*
AP height, mV	50.4 ± 13	50.3 ± 12	−5.5 ± 12†	−5.6 ± 12†	−6.6 ± 16	−2.8 ± 10
AP width, ms	3.3 ± 1	3.1 ± 1	0.3 ± 1†	0.3 ± 0.7†	0.4 ± 1	0.2 ± 0.3
AHP depth, mV	14.1 ± 4	14.0 ± 5	1.1 ± 3†	0.8 ± 3†	1.4 ± 4	0.5 ± 2
AHP 1/2 decay, ms	51.6 ± 30	50.7 ± 30	3.8 ± 15	3.0 ± 15	0.4 ± 17	5.9 ± 15
Slope SS, Hz/nA	94.8 ± 30	99.1 ± 29	4.8 ± 52	12.2 ± 52	12.5 ± 59	−2.4 ± 5

↑, depolarization (≥2 mV in average); ↓, hyperpolarization (≤−2 mV in average);

^†, significant difference with P < 0.05 and

^*, P < 0.005 performed by paired two-sample t-test.