Table 1.

Primer sequences for PCR amplifications

Primer	Sequence	Expected Product Size, bp	Annealing Temperatures
TRPC1 forward	5′-GGTATTTTCTGTGAGAAG-3′	483	Ramped from 37–45° C, 1° C/30 sec.
TRPC1 reverse	5′-CTTCTGATAATTCTCGTC-3′
TRPC3 forward	5′-TCCTATGACGAAGACGGC-3′	600	48°C
TRPC3 reverse	5′-ACAGTTTGGATGAGCCAC-3′
TRPC4 forward	5′-GCTGCTCCTAAGTTTTAATG-3′	618	44°C
TRPC4 reverse	5′-CCAGTTTCAATCTTGCAAG-3′
TRPC5 forward	5′-CGCTTTGGTATGACGGTTTT-3′	178	48°C
TRPC5 reverse	5′-CCGGTGTGGCAGATAAACTT-3′
TRPC6 forward	5′-GATGAAGATGGAACACGG-3′	450	45°C
TRPC6 reverse	5′-GCACTTTGGATGTGCTAC-3′
TRPC7 forward	5′-GAGCTCCTCCTCAAAAAGG-3′	476	47°C
TRPC7 reverse	5′-CTAGTCTGGCCAATTCATTG-3′
SphK1 forward	5′-GAACCATACCACCCATCAG-3′	221	54°C
SphK1 reverse	5′-TGCAGTTGGTCAGCAGTTTC-3′
S1P3R forward	5′-GCCATTCTGGTAGCCATTGT-3′	156	53°C
S1P3R reverse	5′-TGACCAACAGGCAATGAAGA-3′

TRPC, transient receptor potential canonical; SphK, sphingosine kinase; S1P, sphingosine-1-phosphate.