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. 2009 Oct 7;29(40):12702–12710. doi: 10.1523/JNEUROSCI.1166-09.2009

Figure 5.

Figure 5.

DA activates CREB-dependent gene transcription. a, Cortical neurons transfected with 4xCRE luciferase were stimulated at 4 DIV with DA (100 μm) or the β-adrenergic agonist, isoproterenol (100 μm), in the absence or presence of 0.1 μm rolipram for 5 h and then harvested for luciferase assay. **Significantly different from untreated neurons, p < 0.005; ***significantly different from DA alone, p < 0.001; ns, not significantly different from untreated control; n = 3 experiments. b, Stimulation by 100 μm DA in the presence of 0.1 μm rolipram was blocked by Bapta-FA (3 mm), AP5 (100 μm), ifenprodil (10 μm), and verapamil (100 μm); this pharmacological profile is identical to that observed with stimulation by cAMP (Fig. 3a,b). The D1 DA receptor antagonist, SCH23390 (30 μm), also completely blocked stimulation by DA+rolipram. ***Significantly different from DA+rolipram, p < 0.001. Ascorbic acid (100 μm) was included to reduce DA oxidation; control experiments demonstrated that ascorbic acid did not stimulate 4xCRE expression (data not shown).