Figure 1.

Identification of mouse and guinea pig OC90. Two-dimensional SDS/PAGE separation of proteins (10 μg dry weight) from isolated otoconia of the mouse (A) and guinea pig (B). Isoelectric focusing was used in the first dimension (left to right) and SDS/PAGE was used in the second dimension (top to bottom). Protein standards (carbonic anhydrase, ovalbumin, and β-galactosidase) were run alone (C) and spiked into the otoconial preparations shown in A and B. Arrows identify OC90. (D) N-terminal sequence of partially purified mouse and guinea pig otoconins were aligned and used to identify an orphan human cDNA, PLA2L.