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Expression of Oc90 in E17.5 mouse embryo otocysts, brain (minus otocysts), and eyes. Total RNA (5 μg) was reverse-transcribed in vitro. cDNA was amplified by using primers Oto1 and Oto2 (marked by arrows in Fig. 4, Upper) or by using primers specific for the β-actin cDNA as the positive control (Lower). +, with RTase; −, without RTase.
