Table 2.
Descriptive statistics of the tested reference genes
| Rps18 | Sdha | Canx | Pgk1 | Actg1 | Ubc | Rpl13a | Actb | |
|---|---|---|---|---|---|---|---|---|
| GenBank ID | GU188049 | GU188053 | GU188051 | GU188052 | GU188050 | GU188054 | GU188056 | GU188055 |
| AMamplification rate | 1.86 | 1.88 | 1.83 | 1.85 | 1.88 | 1.86 | 1.85 | 1.82 |
| CVamplification rate | 0.06 | 0.05 | 0.05 | 0.05 | 0.06 | 0.05 | 0.05 | 0.05 |
| GMCt-value | 14.62 | 15.37 | 16.63 | 15.72 | 16.83 | 15.72 | 26.05 | 16.05 |
| AMCt-value | 14.64 | 15.42 | 16.68 | 15.78 | 16.88 | 15.79 | 26.10 | 16.16 |
| CVCt-value | 0.04 | 0.07 | 0.07 | 0.07 | 0.07 | 0.08 | 0.06 | 0.09 |
| MinimumCt-value | 13.86 | 13.90 | 14.65 | 13.42 | 14.94 | 12.87 | 24.30 | 13.64 |
| MaximumCt-value | 16.74 | 17.68 | 19.61 | 18.62 | 19.68 | 18.26 | 28.87 | 20.50 |
| SDCt-value | 0.65 | 1.09 | 1.09 | 1.10 | 1.11 | 1.26 | 1.45 | 1.49 |
| SD-threshold | 1.12 | 1.10 | 1.15 | 1.13 | 1.10 | 1.12 | 1.13 | 1.16 |
| Minimum [x-fold] | -1.60 | -2.52 | -3.30 | -4.10 | -3.29 | -5.90 | -2.94 | -4.24 |
| Maximum [x-fold] | 3.71 | 4.27 | 6.10 | 5.93 | 6.00 | 4.84 | 5.65 | 14.49 |
| SD [± x-fold] | 1.50 | 1.99 | 1.99 | 2.00 | 2.01 | 2.21 | 2.49 | 2.56 |
Arithmetic mean (AM), geometric mean (GM), coefficient of variance (CV) and standard deviation (SD) of the amplification rate E and the Ct-values for every potential reference gene. The genes are ordered by their SDCt-value. Genes that showed a SDCt-value smaller than the SD-threshold are considered to be suitable reference genes and marked in bold. The last three rows show the maximum and minimum values of the over- and under-expression of a gene in relation to its calculated geometric mean (displayed as x-fold ratio) as well as the standard deviation (calculated with BestKeeper).