Table 1.
Quantification of phosphorylations on TBP interactors in cell cycle-blocked cells.
| Protein | Peptide | Position | Phospopeptide ratio (G2/M:AS) | Protein ratio (G2/M:AS) | Phosphopeptide/Protein ratio (G2/M:AS) | Reference |
|---|---|---|---|---|---|---|
| TAF1 | TpGMDSNWWVLK | T876 | 150.91 | 1.40 | 107.99 | this study |
| TAF3 | RPLDSpPEAEELPAMK | S183 | 1.38 | 1.60 | 0.86 | this study; ref [31] |
| TAF4 | SpPGVQPQLVLGGAA- QTASLGTATAVQTGTPQR |
S543 | 3.13 | 1.42 | 2.20 | this study |
| TAF7 | YIESpPDVEKEVK | S159 | 15.77 | 1.36 | 11.58 | this study |
| TAFI41/TAF1D | LAGDSpFIVSSEFPVR | S234 | > 200 | 2.88 | > 200 | this study |
| Protein | Peptide | Position | Phospopeptide ratio (G1/S:AS) | Protein ratio (G1/S:AS) | Phosphopeptide/Protein ratio (G1/S:AS) | Reference |
| TAF3 | RPLDSpPEAEELPAMK | S183 | 0.11 | 0.12 | 0.94 | this study; ref [31] |
| TAF3 | QIQTpPPDAGK | T364 | 0.05 | 0.12 | 0.39 | this study |
| TAF3 | RISpGPECTTPK | S427 | 0.02 | 0.12 | 0.19 | this study |
| TAF6 | AAAPPQPSpPPPTK | S532 | 0.07 | 0.11 | 0.62 | this study |
| TAF6 | QEAGDSpPPPAPGTPK | S653 | 0.12 | 0.11 | 1.13 | this study; ref [32] |
| TAF7 | YIESpPDVEKEVK | S159 | 0.11 | 0.17 | 0.63 | this study |
| TAFI41/TAF1D | SRGSGFPFLESpENEK | S137 | 1.43 | 1.35 | 1.06 | this study |
| TAFI41/TAF1D | TQCIPYSpPKGEK | S40 | 1.94 | 1.35 | 1.44 | this study |
Phosphorylations (indicated with a 'p' behind the modified residue) detected on TBP interactors were quantified in cell cycle-blocked cells relative to asynchronous cells and were corrected for protein levels. The sequence of the tryptic peptide detected is indicated.