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Development of fluorescence in the antibody-catalyzed retro-aldol reaction of methodol (Left) and dimedol (Right). Conditions: 200 μM methodol, 6 μM antibody 38C2 in PBS, pH 7.4, and 10% CH3CN; 200 μM dimedol, 10 μM antibody 38C2 in PBS, pH 7.4, and 10% CH3CN. The pictures were taken after the indicated time intervals. The wells were irradiated with a standard long-wave UV lamp.
