Table 3.
Primers used in this study
| Primer Name | Primer Sequence (5' to 3') |
|---|---|
| rhlASVF | GCTCTAGAAGACGGTCATCCTCGTGAAC1 |
| rhlASVR | GGGGTACCCGGCAGCTTCGTCAGATAC1 |
| rhlA1PF | GGAAATGGTCGATGGGTATG2 |
| rhlA2PF | GGCGACGGATAGCGATAAG2 |
| rhlAR | TCGTGTACTCGTCCAGCTC |
| rhlATp1F | GGCGGAATTCCGGCAGGTACTGCTCCGGCCGCATCGACAGGATCTGGTCCGAGCTCGAATTAGCTTCAAA |
| rhlATp1R | TGCCGCGGATCATGAAGCTGTACAACTACCGGTATCTGACGAAGCTGCCGGAGCTCGAATTGGGGATCTT |
| rhlA5'2F | GTGGTCGTGAAAGCGGAAT |
| rhlA5'2R | CGGCAGCTTCGTCAGATAC |
| rhlA3'3F | GACCAGATCCTGTCGATGC |
| rhlA3'3R | CTCGATCAGCGTCATCAGC |
1 Restriction sites designed into the primers are underlined.
2 Primers are constructed upward of the consensus sequence of the two promoters.