Figure 1.

Background Estimation. Using the intensity (y-axis) of the set of low target-affinity probes (ΔG < -3.6 kcal/mol) over the complete set of samples in each experiment (x-axis), the median value approximates the scanner's lower limit specification: the value is 189 f.u. for the Bhattacharjee experiment arrays and 204 f.u. for the Stearman experiment arrays. The Stearman dataset has one obvious outlier (the final sample) which was detected and subsequently removed through our sample cleansing routines.