Figure 5.

(A) Z-DEVD-fmk blocks FTI-induced apoptotic death in KNRK cells. KNRK cells were treated with DMSO (Control), 20 μM SCH56582 (FTI), 20 μM SCH56582 plus 20 μM Z-DEVD-fmk, or 20 μM SCH56582 plus 50 μM Z-DEVD-fmk in 0.1% FCS medium. The number of dead cells was determined 24 h after the treatment by counting the number of trypan blue-stained cells in 100–200 randomly selected cells. Bars = SD. (B) Introduction of the CASP-3 gene restores FTI-induced apoptosis to MCF7 cells. MCF7-vector (stable MCF7 transformants that express GFP) and MCF7-CASP-3 (stable MCF7 transformants that express GFP fused with the wild-type human caspase 3) cells were treated with 150 μM BMS191563 (FTI) for 24 h in 0.1% FCS medium. The cells were analyzed by PI staining and flow cytometry as described in Materials and Methods. Apoptotic cells with a DNA content of <2 N are marked by brackets. At least two separate experiments were carried out with results similar to those shown here.