Figure 6.

Cytochrome c release from mitochondria into cytosol of FTI-treated KNRK but not NRK cells. KNRK (A) or NRK (B) cells were treated with 100 μM BMS191563 in 0.1% FCS medium for the time intervals indicated. Cells were harvested and cytosolic and mitochondrial fractions were prepared. The levels of cytochrome c and actin were determined by Western blot analysis as described in Materials and Methods with the use of monoclonal anti-cytochrome c and anti-actin antibodies, respectively. At least two separate experiments were carried out with results similar to those shown here.