Table 1.

Induction of apoptosis by a variety of FTI in Spon 8 cells

Compound	Cell line	% cells with <2 N DNA content
DMSO (control)	Spon 8	4.7
SCH56582 (FTI)	Spon 8	66.3
BMS191563 (FTI)	Spon 8	62.4
B1088 (FTI)	Spon 8	35.5
FTI-277 (FTI)	Spon 8	27.6

Spon 8 cells were incubated in media containing 0.1% FCS and treated with DMSO, 20 μM SCH56582, 100 μM BMS191563, 80 μM B1088, or 80 μM FTI-277 for 36 h. The cells were analyzed by propidium iodide staining and flow cytometry as described in Materials and Methods. 10,000 cells were examined in each experiment. Apoptotic cell populations were analyzed by using CELL-Quest software (Becton Dickinson Immunocytometry Systems). At least two separate experiments were carried out with results similar to those shown here.