Table 2.

Induction of apoptosis by FTI but not inhibitors of Ras signaling in KNRK cells

Compound	Cell line	% cells with <2 N DNA content
DMSO (control)	KNRK	0.9
SCH56582 (FTI)	KNRK	56.3
Wortmannin	KNRK	0.9
PD98059	KNRK	3.3

KNRK cells were treated with DMSO, 20 μM SCH56582, 1 μM wortmannin KY12420 (phosphatidylinositol 3-kinase inhibitor), or 20 μM PD98059 (MEK inhibitor) for 24 h in 0.1% FCS medium. The cells were analyzed by propidium iodide staining and flow cytometry as described in Materials and Methods; 10,000 cells were examined in each experiment. Apoptotic cell populations were analyzed by using CELL-Quest software (Becton Dickinson Immunocytometry Systems). At least two separate experiments were carried out with results similar to those shown here.