Fig. 3.

Fidelity of incorporation of reversible and irreversible terminators by REAP-42 and REAP-58 using the following primer and templates (polymerases were purified by Qiagen Ni-NTA Spin Kit and quantified according to Bradford assay):

Primer (24 nt): 5′-GCGTAATACGACTCACTATGGACG-3′.

Temp A (36 nt): 3′-CGCATTATGCTGAGTGATACCTGCAATGTGCTTCTG-5′.

Temp G (36 nt): 3′-CGCATTATGCTGAGTGATACCTGCGGTGTGCTTCTG-5′.

Temp T (36 nt): 3′-CGCATTATGCTGAGTGATACCTGCTTTGTGCTTCTG-5′.

Temp C (36 nt): 3′-CGCATTATGCTGAGTGATACCTGCCCTGTGCTTCTG-5′.