Fig. 3.

In vivo silencing of Factor VII in mice. A) Top-performing lipidoids from in vitro screen were purified, formulated for serum stability and delivered intravenously to C57BL/6 mice. Mice received a single bolus administration of 3 mg/kg total siRNA via tail-vein injection and Factor VII levels were quantified 72 h postinjection. B–D) Dose response experiments with top three performing lipidoids from in vivo screen; C16-96 (B), C14-110 (C), and C12-200 (D). No lipidoid-related toxicity is observed as measured by body-weight loss (E). (s.d.,n = 3 or 4, * P < 0.005, **P < 0.001; t-test, single tailed)