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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Microbiol. 2009 Sep 2;12(1):67–83. doi: 10.1111/j.1462-5822.2009.01379.x

Fig. 6. Suppression of Ec-gp96 expression prevents E. coli–induced NO production in HBMEC.

Fig. 6

(A) HBMEC at 30% confluence were transfected with control-siRNA or Ec-gp96-siRNA and allowed to grow to confluence for three days. The cells were tested for the production of NO upon infection with OmpA+ or OmpA− E. coli or treatment with NONOate. (B) Ec-gp96-siRNA/HBMEC or control-siRNA/HBMEC were subjected to binding and invasion assays with E. coli or GBS. The results are expressed as percent binding to or invasion being taken the binding/invasion in control cells as 100%. (C) HBMEC monolayers subjected to various treatments were analyzed for the expression of Ec-gp96 by flow cytometry as described in methods section. All experiments were performed at least three times in triplicate. The increase or decrease in binding to or invasion and the expression of Ec-gp96 was significantly different in comparison to control, *P<0.001 by Student’s t test.