Fig. 8. Overexpression of C-terminal truncated Ec-gp96 proteins in HBMEC prevent the production of NO, decrease the TEER, and increase the permeability induced by OmpA⁺ E. coli.

HBMEC transfected with plasmid alone (pHBMEC) or plasmids containing C-terminal truncations of Ec-gp96 as described in Fig. 7 were infected with OmpA⁺ E. coli for varying periods. The concentrations of NO in the supernatants were determined by Griess reagent (A). Both TEER (B) and HRP leakage (C) were assessed using the transfectant HBMEC monolayers after infecting with OmpA⁺ E. coli. The experiments were performed three times in triplicate. The error bars represent SD.