Table 1.

Purification table

Fractionation step	Activity*, units	Protein, mg	Specific activity, units/mg	Recovery, %
Resource Q flow through	32,000	300	107	100
Ammonium sulfate precipitation	16,667	133	125	52
Resource phenyl	15,000	55	273	47
Mono S	4,670	1.8	2,594	15
Superdex-75	2,500	0.9	2,778	8
Mono Q	250	0.2	1,250	0.8

*The activity was estimated based on a visual scoring system. Six minutes after each reaction was started, the sample was evaluated by fluorescence microscopy. The activity was defined as the amount of the rhodamine-labeled actin around the beads, judged by the fluorescence intensity, with an increasing scale from 1 to 5 units. 0 units indicates that there was no localized rhodamine signal.