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SORD is present along the entire length of sperm flagellum, but does not show the same distribution pattern as α-tubulin. Indirect immunofluorescence of sperm probed with anti-SORD antibody (A), normal goat IgG (B), monoclonal antibody to α-tubulin (C), and normal mouse IgG (D). E) Merged image of A and C. F) Merged image of B and D. G) Corresponding Nomarski differential interference contrast image of test group. H) Corresponding Nomarski image of control group. Bar = 10 μm.
