FIG. 3.

Spontaneous electrical slow waves are coupled to phasic contractions of the myosalpinx. A and B) Simultaneous recordings of slow waves and oviduct contractions. Note that each electrical slow wave (A) precedes a contraction (B) of the myosalpinx (arrows). C) Networks of KIT-positive cells (ICC-OVI) were observed in the myosalpinx (arrows). Bar = 50 μm. D) RT-PCR confirmed expression of Kit in the oviduct, and levels were comparable to expression in muscles of the proximal colon (P. Colon), where ICC are known to be pacemakers [14]. Gapdh was used as a housekeeping control, and NTC represents nontemplate control. Units of measurement for data in the gels are bp.