FIG. 2.

Results of E₂-negative feedback test conducted during the prepubertal period (∼20 wk of age). Left: circulating patterns of LH from two control (two top panels), two prenatal testosterone (T)-treated (two middle panels), and two prenatal DHT-treated (two bottom panels) females during the pretreatment (Pre, phase 0), end of GnRH-A treatment (phase 1), 48 and 72 h after cessation of the GnRH-A treatment (Post-48 h: phase 2; Post-72 h: phase 3), and 3 days postinsertion of E₂ implant (Post-E₂: phase 4). Inverted triangles represent pulses identified by the Cluster algorithm. Right: mean ± SEM number of pulses (top), mean circulating concentrations (middle), and mean pulse amplitude (bottom) of LH in control (open bar), prenatal testosterone-treated (black bar), and prenatal DHT-treated females (hatched bar) during the different phases of E₂-negative feedback test. Different letters (a vs. b and a′ vs. b′) above bars indicate significant differences (P < 0.05). LH pulses were suppressed after GnRH-A treatment in all groups (No Pulses). Two controls and one prenatal testosterone-treated females had no LH pulses throughout the E₂-negative feedback test (phases 0–4) and were not included in determination of pulse attributes.