Left: Mean ± SEM of circulating E2, time of E2 rise, and time interval between E2 rise to LH surge peak in control (white), prenatal testosterone-treated (black), and prenatal DHT-treated (hatched) females during the breeding season following estrus synchronization with PGF2α. Asterisks indicate significant differences (P < 0.05). Middle and right: Mean ± SEM of LH and primary FSH surges peak, timing of gonadotropin surge peak, and interval between start and peak level of gonadotropin achieved during the breeding season following PGF2α synchronization of estrus. Controls are shown as white bars, prenatal testosterone-treated as black bars, and prenatal DHT-treated as hatched bars. Asterisks indicate significant differences (P < 0.05). Data from females that did not synchronize were not included in the analysis.