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. 2008 Dec 23;80(4):771–780. doi: 10.1095/biolreprod.108.072843

FIG. 1.

FIG. 1.

Testicular hyperthermia results in activation of MAPK14 but not MAPK8. A) Time course of activation of MAPK14 and MAPK8 after heat stress, as measured by the TiterZyme Enzyme EIA Kit. Activation of MAPK14, as evidenced by a significant (P < 0.05) increase in phospho-MAPK14, was detected at 0.5, 2, and 6 h after heating. Testicular hyperthermia, on the contrary, had no effect on activation of MAPK8. Values are means ± SEM. Means with unlike letters are significantly (P < 0.05) different. B) Co-staining for TUNEL and phospho-MAPK14 show activation of MAPK14 only in those germ cells undergoing apoptosis at stage XII 6 h after heat treatment (I–III). Green, TUNEL; red, phospho-MAPK14; yellow, colocalization between TUNEL and phospho-MAPK14. Portion of a stage XII tubule from a heat-treated rat pre-incubated with phospho-MAPK14 blocking peptide shows abolition of phospho-MAPK14 immunoreactivity (IV). Bar = 25 μm.